Dataset: Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015

Abstract

Background: Mitochondria play several crucial roles in the life of oligodendrocytes. During development of the myelin sheath they are essential providers of carbon skeletons and energy for lipid synthesis. During normal brain function their consumption of pyruvate will be a key determinant of how much lactate is available for oligodendrocytes to export to power axonal function. Finally, during calcium-overload induced pathology, as occurs in ischemia, mitochondria may buffer calcium or induce apoptosis. Despite their important functions, very little is known of the properties of oligodendrocyte mitochondria, and mitochondria have never been observed in the myelin sheaths.

Methods and results: We have used targeted expression of fluorescent mitochondrial markers to characterize the location and movement of mitochondria within oligodendrocytes. We show for the first time that mitochondria are able to enter and move within the myelin sheath. Within the myelin sheath the highest number of mitochondria was in the cytoplasmic ridges along the sheath. Mitochondria moved more slowly than in neurons and, in contrast to their behavior in neurons and astrocytes, their movement was increased rather than inhibited by glutamate activating NMDA receptors. By electron microscopy we show that myelin sheath mitochondria have a low surface area of cristae, which suggests a low ATP production.

Conclusion: These data specify fundamental properties of the oxidative phosphorylation system in oligodendrocytes, the glial cells that enhance cognition by speeding action potential propagation and provide metabolic support to axons.

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Variable Groups

Full Title

Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015

Identification Number

NSD2289

Authoring Entity

Name Affiliation
Rinholm, Johanne Egge Department of Anatomy, Faculty of Medical Sciences, University of Oslo

Producer

Name Affiliation Abbreviation Role
Rinholm, Johanne Egge Department of Anatomy, Faculty of Medical Sciences, University of Oslo UiO Principal Investigator

Copyright

Copyright (C) 2015 Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, UiO

Funding Agency/Sponsor

Name Abbreviation Role Grant
The Research Council of Norway RCN 213690

Data Distributor

Name Affiliation Abbreviation
NSD - Norwegian Centre for Research Data NSD

Version

Date: 2016-01-28

Notes

Original data from Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, University of Oslo are documented and prepared, first NSD-version.

Bibliographic Citation

"Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015". Data collected by Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, University of Oslo. First NSD edition, Bergen 2016.

List of Keywords

Topic Classification

Time Period Covered

Start End Cycle
2012-01-01 2015-12-01

Date of Collection

Start End Cycle
2012-12-01 2015-12-01

Country

Norway  (NO)

Geographic Coverage

Country

Unit of Analysis

Other

Universe

Geographical localization of mitochondria in oligodendrocytes, density, size, proportion mobile mitochondria, speed of mobile mitochondria.
Data about cell anatomy and function in mouse brains.

Kind of Data

Other

Time Method

Cross-sectional survey

Data Collector

Rinholm, Johanne Egge, Department of Geosciences, University of Oslo  (UiO)

Sampling Procedure

Collection method: microscopic examination.

Gross sample: -1
Net sample: 5

Mode of Data Collection

Other

Location

Availability Status

Data from "Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015" are made available for everyone when ordered.

Extent of Collection

2 data file(s); different formats can be made.

Restrictions

Data from ”Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015” are made available for everyone when ordered.

Citation Requirement

Users are obliged to refer to producer and distributor of the data by writing the following in forewords or footnotes in eventual publications:
"(Some of) the data applied in the analysis in this publication are based on "Why Oligodendrocytes Keep Axons Alive, but Easily Die, 2015". The survey was financed by The Reasearch Council of Norway. The data are provided by Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, University of Oslo and prepared and made available by Norwegian Centre for Research Data . Neither Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

Deposit Requirement

Access to data is given on the condition that NSD gets a PDF-file of eventual reports that are written on the basis of the data. This to ensure best possible information of the use of data we distribute.

Conditions

The order form has to include name, institutional affiliation, project title, information about sources of financing and postal address. A declaration of secrecy has to be signed before delivery of data.

Disclaimer

Neither Johanne Egge Rinholm, Department of Anatomy, Faculty of Medical Sciences, University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

Related Publications

Movement and Structure of Mitochondria in Oligodendrocytes and Their Myelin Sheaths

Rinholm, J. E.,  Vervaeke, K.,Tadross, M. R. Tkachuk, A. N., Kopek, B. G., Brown, T. A., Bergersen, L. H. and Clayton, D. A. (2016) Movement and Structure of Mitochondria in Oligodendrocytes and Their Myelin Sheaths. Glia 2016, 17 January. Wiley Periodicals, Inc.

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