Dataset: Metabolic Enzymes in Glial Cells of the Honeybee Brain and Their Associations with Aging, Starvation and Food Response, 2017

Download

Abstract

The honey bee has been extensively studied as a model for neuronal circuit and memory function and more recently has emerged as an unconventional model in biogerontology. Yet, the detailed knowledge of neuronal processing in the honey bee brain contrasts with the very sparse information available on glial cells. In other systems glial cells are involved in nutritional homeostasis, detoxification, and aging. These glial functions have been linked to metabolic enzymes, such as glutamine synthetase and glycogen phosphorylase. As a step in identifying functional roles and potential differences among honey bee glial types, this project examined the spatial distribution of these enzymes and asked if enzyme abundance is associated with aging and other processes essential for survival. Using immunohistochemistry and confocal laser microscopy we demonstrate that glutamine synthetase and glycogen phosphorylase are abundant in glia but appear to co-localize with different glial sub-types. The overall spatial distribution of both enzymes was not homogenous and differed markedly between different neuropiles and also within each neuropil. Using semi-quantitative Western blotting it was found that rapid aging, typically observed in shortest-lived worker bees (foragers), was associated with declining enzyme levels. Further, enzyme abundance changes after severe starvation stress, and glutamine synthetase is associated with food response. Together, the data indicate that aging and nutritional physiology in bees are linked to glial specific metabolic enzymes. Enzyme specific localization patterns suggest a functional differentiation among identified glial types.

The data material consists of four files. The spreadsheat data allows to identify for each blot and lane the experiment/treatment.
- Experiment Starvation with ST=starved and SA=satiated for Fig. 4
- Experiment gustatory responsiveness/GRS with L=low and H=high responders for Fig. 5
- Experiment Aging with OF=old and YF=young foragers for Fig. 6

Data are freely available for downloading.

Variable Groups

Full Title

Metabolic Enzymes in Glial Cells of the Honeybee Brain and Their Associations with Aging, Starvation and Food Response, 2017

Identification Number

NSD2579

Authoring Entity

Name Affiliation
Münch, Daniel Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences

Producer

Name Affiliation Abbreviation Role
Münch, Daniel Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences MINA, NMBU Principal Investigator

Copyright

Copyright (C) 2018 Daniel Münch, MINA, NMBU

Data Distributor

Name Affiliation Abbreviation
NSD – Norwegian Centre for Research Data NSD

Version

Date: 2018-06-12

Notes

Original data from Daniel Münch, MINA, NMBU are documented and prepared, first NSD-version.

Bibliographic Citation

"Metabolic Enzymes in Glial Cells of the Honeybee Brain and Their Associations with Aging, Starvation and Food Response, 2017". Data collected by Daniel Münch, MINA, NMBU. First NSD edition, Bergen 2018.

List of Keywords

Topic Classification

Time Period Covered

Start End Cycle
2011 2017

Date of Collection

Start End Cycle
2011-08-30 2017-09-30

Country

Norway  (NO)

Unit of Analysis

Other

Universe

Honey bees

Kind of Data

Other

Time Method

Cross-sectional survey

Data Collector

Münch, Daniel, Faculty of Environmental Sciences and Resource Management, Norwegian University of Life Sciences  (MINA, NMBU)

Mode of Data Collection

Other

Location

Availability Status

Data are freely available for downloading.

Extent of Collection

4 data files; different formats can be made.

Restrictions

Data are freely available for downloading.

Citation Requirement

Users are obliged to refer to producer and distributor of the data by writing the following in forewords or footnotes in eventual publications:
“(Some of) the data applied in the analysis in this publication are based on "Metabolic Enzymes in Glial Cells of the Honeybee Brain and Their Associations with Aging, Starvation and Food Response, 2017". The data are provided by Daniel Münch, and prepared and made available by the NSD – Norwegian Centre for Research Data. Neither Daniel Münch, MINA, NMBU, The Research Council of Norway nor NSD are responsible for the analyses/interpretation of the data presented here.”

Disclaimer

Neither Daniel Münch, MINA, NMBU, The Research Council of Norway nor NSD are responsible for the analyses/interpretation of the data presented here.

Related Materials

Western blot data for semiquantitative analyses

PPT file for Figs 1-3

Related Publications

Metabolic enzymes in glial cells of the honeybee brain and their associations with aging, starvation and food response

Ashish K. Shah, Claus D. Kreibich, Gro V. Amdam and Daniel Münch (2018) Metabolic enzymes in glial cells of the honeybee brain and their associations with aging, starvation and food response, Journal: PLOS ONE Accepted/publication year: 2018

Metadata Index

This is the Metadata Index for a Nesstar Server.
Nesstar is a tool used for analysing, visualising and downloading datasets.

Click the "Explore Dataset" button to open the dataset.