Dataset: Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015

Abstract

The data presented are collected through the project “Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015”. Mycobacterium tuberculosis (M.tb), the cause of tuberculosis (TB) infects one-third of the world population, one person per second, and kills about 2 million people per year. The only effective therapy, using antibiotics, is being challenged by increasing antibiotic resistance. It has been shown that when antibiotics are encapsulated in a biodegradable nanoparticle (NP) made from a polymer such as poly (lactate-co-glycolate) acid (PLGA) the drug is significantly more effective in curing TB in different animal models; higher concentrations of antibiotics can be targeted selectively to macrophages, the infection site, where they are released slowly, while the total systemic levels is much less than with standard drug therapy. A weakness of the existing animal models of TB is the difficulty in visualizing the NPs in the infected live animal, which is essential if one is to optimize beads for their ability to reach infected cells after different modes of administration. This is precisely the great strength of the transparent zebrafish model of tuberculosis based on red and green variants of M.marinum, the fish TB organism. In this project a model using red and green fluorescent M. marinum was established, and it also successfully fabricated PLGA NPs enclosing the antibiotic rifampicin and red or green fluorescent dyes. When these beads are injected into embryos 1-2 days after infection they reach the same cells in the granulomas as the bacteria and we have preliminary data giving hints of therapy. Furthermore the aim is now to perfect this system for embryos and adults and find the most effective NPs that reach granulomas and provide a robust therapy. This system enables to screen for different kinds of NPs, as well as to compare different antibiotics and combinations thereof, and cDNA's coding for anti-microbial peptides. The most effective NPs will be given to collaborators in India to test them in a guinea pig model as a prelude to testing the NPs in humans.

For access to the dataset, use the NSD application form:  
http://www.nsd.uib.no/nsd/english/orderform.html

Variable Groups

Full Title

Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015

Identification Number

NSD2247

Authoring Entity

Name Affiliation
Griffiths, Gareth Department of Biosciences, University of Oslo

Producer

Name Affiliation Abbreviation Role
Griffiths, Gareth Department of Biosciences, University of Oslo UiO Principal Investigator

Copyright

Copyright (C) 2015 Gareth Griffiths, Department of Biosciences, UiO

Funding Agency/Sponsor

Name Abbreviation Role Grant
The Research Council of Norway RCN 213830

Data Distributor

Name Affiliation Abbreviation
NSD - Norwegian Centre for Research Data NSD

Version

Date: 2015-11-14

Notes

Original data from Gareth Griffiths, Department of Biosciences, University of Oslo are documented and prepared, first NSD-version.

Bibliographic Citation

"Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015". Data collected by Gareth Griffiths, Department of Biosciences, University of Oslo. First NSD edition, Bergen 2015.

List of Keywords

Topic Classification

Time Period Covered

Start End Cycle
2012 2015

Date of Collection

Start End Cycle
2012-08-01 2015-07-01

Country

Norway  (NO)

Geographic Coverage

Country

Unit of Analysis

Other

Universe

Zebrafish embryos

Kind of Data

Other

Time Method

Cross-sectional survey

Data Collector

Griffiths, Gareth, Department of Biosciences, University of Oslo  (UiO)

Sampling Procedure

Biological data from animal research. The mode of data collection is microscopic imaging (transmission electron, confocal and fluorescent stereo microscopy) and biological assays (CFU/time-kill, CCK8, crystal violet, fluorescent pixel count, survival analysis, in vivo toxicity analysis).

Mode of Data Collection

Other

Location

Availability Status

Data from ”Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015” are made available for everyone when ordered.

Extent of Collection

54 data files; different formats can be made.

Restrictions

Data from "Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015" are made available for everyone when ordered.

Citation Requirement

Users are obliged to refer to producer and distributor of the data by writing the following in forewords or footnotes in eventual publications:
"(Some of) the data applied in the analysis in this publication are based on "Development of Nanoparticle Based Therapies Against Tuberculosis in the Zebrafish Model, 2015". The survey was financed by The Reasearch Council of Norway. The data are provided by Gareth Griffiths, Department of Biosciences, University of Oslo and prepared and made available by NSD - Norwegian Centre for Research Data. Neither Gareth Griffiths, Department of Biosciences, University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

Deposit Requirement

Access to data is given on the condition that NSD gets a PDF-file of eventual reports that are written on the basis of the data. This to ensure best possible information of the use of data we distribute.

Conditions

The order form has to include name, institutional affiliation, project title, information about sources of financing and postal address. A declaration of secrecy has to be signed before delivery of data.

Disclaimer

Neither Gareth Griffiths, Department of Biosciences, University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

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