Dataset: Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016

Abstract

One third of the world's population is infected with Mycobacterium tuberculosis (M.tb) and two million die annually of tuberculosis (TB). The standard therapy, using antibiotics, is increasingly ineffective due to multi-drug resistance. The only available, 80 year-old vaccine using M.bovis BCG has little therapeutic value, and no other vaccines have reached the market, despite enormous efforts. This project proposed to develop technology to encapsulate whole mycobacteria inside biodegradable nano/microbeads to facilitate delivery of intact live or killed bacteria to vertebrate antigen-presenting cells (APC) to initiate the immune response.  

The Oslo nano-bead group of Nystrom investigated different polymer materials that could encapsulate live and killed myco bacteria, focusing on the fish TB bacterium M.marinum, BCG and the mouse/human pathogen M.avium and Mycobacterium w, a promising antituberculous vaccine candidate. The Oslo cell microbiology group of Griffiths has established a system using GFP-M.marinum lethal infection of transparent zebrafish. After first experiments with BCG- and M.avium-beads in macrophages in vitro we searched for the most effective GFPbacteria-enclosing beads that when injected or fed to zebrafish crosses mucosal barriers and induces protection against subsequent infection with M.marinum. This rapid fish screening system would narrow down the promising polymer candidates/conditions for testing beads enclosing M.avium, Mw and later virulent M.tb, for their vaccine potential in mice and guinea pigs against subsequent challenge with these pathogens by Verma and Khuller in India. Different oral, intranasal and subcutaneous vaccination routes were planned to be tested. A crucial advantage of nanobeads over freely-administered immunogens is their established ability to cross mucosal barriers intact. This strategy ensures that the first location where mycobacteria become exposed to antigen digestion/ presentation systems is in the phago-lysosomes of APC's.

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Variable Groups

Full Title

Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016

Identification Number

NSD2354

Authoring Entity

Name Affiliation
Griffiths, Gareth The Faculty of Mathematics and Natural Sciences, University of Oslo

Producer

Name Affiliation Abbreviation Role
Griffiths, Gareth The Faculty of Mathematics and Natural Sciences, University of Oslo UiO Principal Investigator

Copyright

Copyright (C) 2016 Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, University of Oslo

Funding Agency/Sponsor

Name Abbreviation Role Grant
The Research Council of Norway RCN 196377

Data Distributor

Name Affiliation Abbreviation
NSD – Norwegian Centre for Research Data NSD

Version

Date: 2016-10-26

Notes

Original data from Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, University of Oslo are documented and prepared, first NSD-version.

Bibliographic Citation

"Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016". Data collected by Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, University of Oslo. First NSD edition, Bergen 2016.

List of Keywords

Topic Classification

Time Period Covered

Start End Cycle
2012 2016

Date of Collection

Start End Cycle
2012-01-01 2015-10-31

Country

Norway  (NO)

Unit of Analysis

Other

Kind of Data

Experimental data

Time Method

Cross-sectional survey

Data Collector

Griffiths, Gareth, The Faculty of Mathematics and Natural Sciences, University of Oslo  (UiO)

Mode of Data Collection

Laboratory observation

Location

Availability Status

NSD can in agreement with the Principal Investigator make data from "Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016" available when ordered.

Extent of Collection

71 data files; different formats can be made.

Restrictions

NSD can in agreement with the Principal Investigator make data from ”Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016” available when ordered.

Citation Requirement

Users are obliged to refer to producer and distributor of the data by writing the following in forewords or footnotes in eventual publications:
"(Some of) the data applied in the analysis in this publication are based on "Development of Biodegradable Nanobeads As Vaccines Against Tuberculosis, 2016". The survey was financed by The Reasearch Council of Norway. The data are provided by Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, the University of Oslo and prepared and made available by NSD – Norwegian Centre for Research Data. Neither Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, the University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

Deposit Requirement

Access to data is given on the condition that NSD gets a PDF-file of eventual reports that are written on the basis of the data. This to ensure best possible information of the use of data we distribute.

Conditions

The order form has to include name, institutional affiliation, project title, information about sources of financing and postal address. A declaration of secrecy has to be signed before delivery of data.

Disclaimer

Neither Gareth Griffiths, The Faculty of Mathematics and Natural Sciences, the University of Oslo, The Research Council of Norway nor NSD are responsible for the analysis/interpretation of the data presented here.

Related Publications

Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system

Løvmo. S.D. Speth, M.T. Repnik, U. Koppang, E.O. Griffiths, G.W. Hildahl, J.P. (2016). Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system, Developmental and Comparative Immunology. http://dx.doi.org/10.1016/

Layer-by-layer nanocoating of live Bacille-Calmette-Guérin mycobacteria with poly(I:C) and chitosan enhances pro-inflammatory activation and bactericidal capacity in murine macrophages

Speth, M. T. Repnik, U. Griffiths, G. (2016). Layer-by-layer nanocoating of live Bacille-Calmette-Guérin mycobacteria with poly(I:C) and chitosan enhances pro-inflammatory activation and bactericidal capacity in murine macrophages, Biomaterials. http://dx.doi.org/10.1016/j.biomaterials.2016.09.027

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